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Rhodamine 123 A fluorescent potentiometric dye that accumulates in mitochondria in a manner which is dependent on membrane polarization

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The method, known as the R2KD assay, was used to characterise binding of NMR fragment screen hit 27 to TRF1 TRFH and revealed a K D value of 280 M (95% confidence intervals (CI): 230350 M, Fig

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